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Recombinant DNA technology(plasmid DNA, Recombinant DNA (ÀçÁ¶ÇÕ DNA), miniprep, cloning, Àü±â¿µµ¿ °á°ú ºÐ¼®) / 1. Title 2. Date 3. Purpose 4. Introduction 5. Material & Method 6. Result 7. Discussion / 1. Title A Comprehensive Examination of Plasmid DNA, Cloning Techniques, and Analysis of Electrophoresis Results Recombinant DNA ±â¼úÀº »ý¸í°úÇÐ ºÐ¾ß¡¦ |
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ºÐ»ý½Ç ºÐÀÚ»ý¹°ÇнÇÇè recombinant plasmid DNA cloning ¿¹ºñ / 1. Subjectrecombinant plasmid DNA cloning 2. Introduction / 1. Subjectrecombinant plasmid DNA cloning Recombinant plasmid DNA cloningÀº À¯ÀüÀÚ Á¶ÀÛÀÇ ÇÙ½É ±â¼ú·Î, ƯÁ¤ DNA Á¶°¢À» Çö󽺹̵å¶ó´Â ¿øÇü DNA ºÐÀÚ¿¡ »ðÀÔÇÏ¿© »õ·Î¿î À¯ÀüÇüÁúÀ» °¡Áø »ý¹°Ã¼¸¦ ¸¸µå´Â °úÁ¤ÀÌ´Ù. ÀÌ °úÁ¤Àº ´Ù¾çÇÑ »ý¹°ÇÐÀû ¡¦ |
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[ÀϹݻý¹°ÇнÇÇè]Expression of recombinant -synuclein gene / 1. ½ÇÇè ¹æ¹ý 2. ½ÇÇè °á°ú 3. ÅäÀÇ »çÇ× 4. Âü°í ¹®Çå / 1. ½ÇÇè ¹æ¹ý ÀÌ ½ÇÇè¿¡¼´Â ÀçÁ¶ÇÕ ¥á-synuclein À¯ÀüÀÚÀÇ ¹ßÇöÀ» ¸ñÇ¥·Î ÇÑ´Ù. ¸ÕÀú, ¥á-synuclein À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÑ Çö󽺹̵å DNA¸¦ ÁغñÇÑ´Ù. À̸¦ À§ÇØ ÇØ´ç À¯ÀüÀÚ¸¦ Ŭ·Î´×ÇÒ º¤Å͸¦ ¼±ÅÃÇÏ°í, È¿À²ÀûÀÎ ¹ßÇöÀ» À§ÇØ T7 ÇÁ·Î¸ðÅÍ°¡ Æ÷ÇÔµÈ »ó¿ë º¤ÅÍ¡¦ |
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À¯ÀüÀÚ ÀçÁ¶ÇÕ ±â¼ú (Recombinant DNA Technology) / ¥°. ¼·Ð ¥±. Àç·á ¹× ¹æ¹ý 1. ½ÇÇèÀç·á 2. ½ÇÇè¹æ¹ý ¥². °á°ú ¥³. ³íÀÇ / ¥°. ¼·Ð À¯ÀüÀÚ ÀçÁ¶ÇÕ ±â¼ú(Recombinant DNA Technology)Àº »ý¸í°úÇÐ ºÐ¾ßÀÇ Çõ½ÅÀûÀÎ ±â¼ú Áß Çϳª·Î, ƯÁ¤ À¯ÀüÀÚ¸¦ ´Ù¸¥ À¯ÀüÀÚ È¤Àº »ý¹°Ã¼¿¡ »ðÀÔÇÏ¿© »õ·Î¿î À¯ÀüÀÚ¸¦ Çü¼ºÇÏ´Â °úÁ¤À» Æ÷ÇÔÇÑ´Ù. ÀÌ ±â¼úÀº 1970³â´ë Ãʹݿ¡ µîÀåÇÏ¿´À¸¸ç¡¦ |
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Àΰø½ÅÀå½Ç óġ ¹× °Ë»ç(º¹¸·ÆòÇü°Ë»ç(PET), Á¶Ç÷Á¦(Recombinant Human Erythropoietin º¸°í¼ / 1) óġ ¹× °Ë»ç °úÁ¦ ¾ç½Ä 2) Åõ¾à °úÁ¦ ¾ç½Ä / 1) óġ ¹× °Ë»ç °úÁ¦ ¾ç½Ä Àΰø½ÅÀå½Ç óġ ¹× °Ë»ç °úÁ¦ ¾ç½ÄÀº ȯÀÚÀÇ Ä¡·á ¹× °ü¸®¿¡ ÇʼöÀûÀÎ Á¤º¸¿Í ÀýÂ÷¸¦ ü°èÀûÀ¸·Î ±â·ÏÇÏ´Â ¹®¼ÀÌ´Ù. ÀÌ ¾ç½ÄÀº °£È£»ç, ÀÇ»ç, ±â¼úÀÚ µî ¸ðµç ÀÇ·áÁøÀÌ °øÀ¯ÇÏ°í Âü°íÇÒ ¼ö ÀÖ´Â ±âº» Àڷᡦ |
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1. ½ÇÇè¸ñÀû À̹ø ½ÇÇèÀÇ ¸ñÀûÀº ÀçÁ¶ÇÕ DNA ±â¼úÀ» ÀÌ¿ëÇÏ¿© ¿Ü·¡ À¯ÀüÀÚ¸¦ ¿¡ Äݸ®(E. coli)¿¡ µµÀÔÇÏ°í, À̷κÎÅÍ Çü±¤ ´Ü¹éÁúÀÎ ³ì.. / 1. ½ÇÇè¸ñÀû 2. ½ÇÇè¿ø¸® 3. ½ÇÇè¹æ¹ý 4. ½ÇÇè°á°ú 5. Âü°í¹®Çå / 1. ½ÇÇè¸ñÀû À̹ø ½ÇÇèÀÇ ¸ñÀûÀº ÀçÁ¶ÇÕ DNA ±â¼úÀ» ÀÌ¿ëÇÏ¿© ¿Ü·¡ À¯ÀüÀÚ¸¦ ¿¡ Äݸ®(E. coli)¿¡ µµÀÔÇÏ°í, À̷κÎÅÍ Çü±¤ ´Ü¹éÁúÀÎ ³ì»ö Çü±¤ ´Ü¹éÁú(Green Fluorescent¡¦ |
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Extraction of various carotenoids from recombinant Escherichia coli °á°úº¸°í¼ / 1. ½ÇÇèÁ¦¸ñ 2. ½ÇÇè¸ñÀû 3. ½ÇÇè¹æ¹ý 4. ½ÇÇè°á°ú 5. °íÂû 6. Âü°í¹®Çå / 1. ½ÇÇèÁ¦¸ñ ½ÇÇèÁ¦¸ñÀº `ÀçÁ¶ÇÕ ´ëÀå±Õ¿¡¼ ´Ù¾çÇÑ Ä«·ÎƼ³ëÀ̵åÀÇ ÃßÃâ`ÀÌ´Ù. º» ½ÇÇèÀº ´ëÀå±Õ(E. coli)À» ÀÌ¿ëÇÏ¿© Ä«·ÎƼ³ëÀ̵带 ÇÕ¼ºÇÏ°í À̸¦ È¿À²ÀûÀ¸·Î ÃßÃâÇÏ´Â °úÁ¤À» Ž±¸ÇÏ°íÀÚ ÇÏ¿´´Ù. Ä«·ÎƼ³ëÀ̵å´Â¡¦ |
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Extraction of various carotenoids from recombinant Escherichia coli ¿¹ºñº¸°í¼ / 1. ½ÇÇè¸ñÀû 2. ½ÇÇèÀÌ·Ð 3. ½ÇÇè¹æ¹ý 4. ½Ã¾àÁ¶»ç 5. Âü°í¹®Çå / 1. ½ÇÇè¸ñÀû Carotenoids´Â ½Ä¹°, ¹Ì»ý¹° ¹× ÀϺΠµ¿¹°¿¡¼ ¹ß°ßµÇ´Â õ¿¬ »ö¼Ò·Î, ±¤ÇÕ¼º¿¡¼ Áß¿äÇÑ ¿ªÇÒÀ» ÇÏ¸ç °·ÂÇÑ Ç×»êÈ Æ¯¼ºÀ» Áö´Ñ´Ù. ÀÌ·¯ÇÑ ¹°ÁúÀº ÀηùÀÇ °Ç°°ú ¿µ¾ç¿¡ Áß¿äÇÑ ¿µÇâÀ» ¹ÌÄ¡¸ç, ½ÄÇ° ¹× ÈÀåÇ° ¡¦ |
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Àΰø½ÅÀå½Ç óġ ¹× °Ë»ç(º¹¸·ÆòÇü°Ë»ç(PET), Á¶Ç÷Á¦(Recombinant Human Erythropoietin)) º¸°í¼ / 1) óġ ¹× °Ë»ç °úÁ¦ ¾ç½Ä 2) Åõ¾à °úÁ¦ ¾ç½Ä / 1) óġ ¹× °Ë»ç °úÁ¦ ¾ç½Ä Àΰø½ÅÀå½Ç óġ ¹× °Ë»ç °úÁ¦ ¾ç½Ä¿¡ ´ëÇØ ¼³¸íÇÏ°Ú´Ù. ÀÌ ¹®¼´Â ȯÀÚÀÇ Ä¡·á °úÁ¤¿¡¼ ¼öÇàµÇ´Â ´Ù¾çÇÑ ÀýÂ÷¿Í °Ë»ç¿¡ ´ëÇÑ ±â·ÏÀ» Æ÷ÇÔÇÑ´Ù. º¹¸·ÆòÇü°Ë»ç(PET)¿Í Á¶Ç÷Á¦ÀÎ ÀçÁ¶ÇÕ Àΰ£ÀûÇ÷±¸»ý¼ºÈ£¸£¡¦ |
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Extraction of various carotenoids from recombinant Escherichia coli °á°úº¸°í¼ / 1. ½ÇÇèÁ¦¸ñ 2. ½ÇÇè¸ñÀû 3. ½ÇÇè¹æ¹ý 4. ½ÇÇè°á°ú 5. °íÂû 6. Âü°í¹®Çå / 1. ½ÇÇèÁ¦¸ñ ½ÇÇè Á¦¸ñÀº `Extraction of Various Carotenoids from Recombinant Escherichia coli`ÀÌ´Ù. ÀÌ ¿¬±¸´Â ÀçÁ¶ÇÕ ´ëÀå±Õ¿¡¼ ´Ù¾çÇÑ Ä«·ÎƼ³ëÀ̵带 ÃßÃâÇÏ°í ºÐ¸®ÇÏ´Â °úÁ¤À» ´Ù·ç°í ÀÖÀ¸¸ç, Ä«·ÎƼ³ëÀ̵塦 |
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