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[A0] ¼°´ë Çö´ë»ý¹°ÇнÇÇè2 4Â÷ Ç®·¹Æ÷Æ® - GFPuv¸¦ ÀÌ¿ëÇÑ Site-directed mutagenesisÀÇ ½Ç½À°ú DNA sequencing °á°úÀÇ ºÐ¼® ¿¬½À / 1. Abstract 2. Introduction 3. Materials & Method 4. Result 5. Discussion 6. Reference / 1. Abstract º» ½ÇÇèÀº GFPuv(Çü±¤ ´Ü¹éÁú)¸¦ ÀÌ¿ëÇÑ site-directed mutagenesis ±â¹ýÀ» Àû¿ëÇÏ¿© ƯÁ¤ À¯ÀüÀÚÀÇ º¯À̸¦ À¯µµÇÏ°í, ÀÌÀÇ ¼¿ ºÐ¡¦ |
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[A0] ¼°´ë Çö´ë»ý¹°ÇнÇÇè2 4Â÷ Ç®·¹Æ÷Æ® - GFPuv¸¦ ÀÌ¿ëÇÑ Site-directed mutagenesisÀÇ ½Ç½À°ú DNA sequencing °á°úÀÇ ºÐ¼® ¿¬½À / 1. Abstract 2. Introduction 3. Materials & Method 4. Result 5. Discussion 6. Reference / 1. Abstract º» ½ÇÇè¿¡¼´Â GFPuv(green fluorescent protein uv) À¯ÀüÀÚ¸¦ ÀÌ¿ëÇÑ Site-directed mutagenesis ±â¹ýÀ» ÅëÇØ Æ¯Á¤ ¾Æ¹Ì³ë»ê Àܱ⸦¡¦ |
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ÀϹݻý¹°ÇнÇÇè GFP´Ü¹éÁú Á¤·® ½ÇÇè / 1.Abstract 2.Introduction (1)´Ü¹éÁú (2)³ì»öÇü±¤´Ü¹éÁú(GFP)°ú EGFP (3)ºÐ±¤±¤µµ¹ý(Spectrophotometry)¿Í Lambert- Beer¡¯s law (4)Extinction coefficient essay (5)Lowry assay (6)Standard curve(Ç¥ÁØ°î¼±) 3.Materials & Methods 4.Result 5.Discussion / 1.Abstract ÀÌ ¿¬±¸¿¡¼´Â ³ì»ö Çü±¤ ´Ü¹éÁú(GFP)ÀÇ Á¤·®È¸¦ ÅëÇØ¡¦ |
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1. ½ÇÇè¸ñÀû À̹ø ½ÇÇèÀÇ ¸ñÀûÀº ÀçÁ¶ÇÕ DNA ±â¼úÀ» ÀÌ¿ëÇÏ¿© ¿Ü·¡ À¯ÀüÀÚ¸¦ ¿¡ Äݸ®(E. coli)¿¡ µµÀÔÇÏ°í, À̷κÎÅÍ Çü±¤ ´Ü¹éÁúÀÎ ³ì.. / 1. ½ÇÇè¸ñÀû 2. ½ÇÇè¿ø¸® 3. ½ÇÇè¹æ¹ý 4. ½ÇÇè°á°ú 5. Âü°í¹®Çå / 1. ½ÇÇè¸ñÀû À̹ø ½ÇÇèÀÇ ¸ñÀûÀº ÀçÁ¶ÇÕ DNA ±â¼úÀ» ÀÌ¿ëÇÏ¿© ¿Ü·¡ À¯ÀüÀÚ¸¦ ¿¡ Äݸ®(E. coli)¿¡ µµÀÔÇÏ°í, À̷κÎÅÍ Çü±¤ ´Ü¹éÁúÀÎ ³ì»ö Çü±¤ ´Ü¹éÁú(Green Fluorescent¡¦ |
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1. Abstract º» ½ÇÇè¿¡¼´Â ³ì»öÇü±¤´Ü¹éÁú(GFP)ÀÇ ¹ßÇö°ú Á¤·®È¸¦ ÅëÇØ ´Ü¹éÁú »ý¹°ÇÐÀÇ ±âº» °³³äÀ» Ž±¸ÇÏ¿´´Ù. GFP´Â Çؾç ÀÚ°³.. / 1. Abstract 2. Introduction 3. Materials and Methods 4. Results 5. Discussion 6. References / 1. Abstract º» ½ÇÇè¿¡¼´Â ³ì»öÇü±¤´Ü¹éÁú(GFP)ÀÇ ¹ßÇö°ú Á¤·®È¸¦ ÅëÇØ ´Ü¹éÁú »ý¹°ÇÐÀÇ ±âº» °³³äÀ» Ž±¸ÇÏ¿´´Ù. GFP´Â Çؾç ÀÚ°³Á¶°³¡¦ |
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GFP ´Ü¹éÁú Á¤·®°ú SDS-PAGE ½ÇÇè ·¹Æ÷Æ® / 1. Abstract 2. Introduction (1) GFP, EGFP (2) Å©·Î¸¶Åä±×·¡ÇÇ (3) SDS-PAGE (4) extinction coefficient assay (5) Lowry assay 3. Material and Method 4. Result (1) elutionµÈ EGFP (2) extinction coefficient assay (3) SDS-PAGE °á°ú (4) BSA standard curve (5) SpectrofluorometerÁ¤·®ÀÇ °á°ú 5. Discussion /¡¦ |
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GFP ´Ü¹éÁú°ú ´Ü¹éÁú Á¤·® A / ¥°. Abstract ¥±. Introduction III. Materials & Methods ¥³. Results ¥´. Discussion ¥µ. Reference / ¥°. Abstract GFP ´Ü¹éÁú°ú ´Ü¹éÁú Á¤·® A¿¡ ´ëÇÑ ¿¬±¸´Â »ý¸í°úÇÐ ¹× »ý¸í°øÇÐ ºÐ¾ß¿¡¼ Áß¿äÇÑ ÁÖÁ¦ÀÌ´Ù. GFP´Â ³ì»ö Çü±¤ ´Ü¹éÁú·Î, ÇØ¾ç »ý¹°ÀÎ Çظ¶¿¡¼ óÀ½ ¹ß°ßµÇ¾úÀ¸¸ç, ÀÌÈÄ ´Ù¾çÇÑ »ý¹°ÇÐÀû ¿¬±¸¿¡ ³Î¸® È°¿ëµÇ¾ú´Ù. GFP´Â ¿ì¼öÇÑ¡¦ |
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GFP ´Ü¹éÁú°ú ´Ü¹éÁú Á¤·®(Protein Measurements) (1) / ¥°. ¿ä¾à(Abstract) ¥±. ¼·Ð (Introduction) ¥². ½ÇÇèÀç·á ¹× ¹æ¹ý(Materials and Methods) ¥³. °á°ú(Results) ¥´. ³íÀÇ (Discussion) ¥µ. Âü°í¹®Çå(References) / ¥°. ¿ä¾à(Abstract) GFP ´Ü¹éÁú(Green Fluorescent Protein)Àº 20¼¼±â ÈĹݿ¡ ¹ß°ßµÈ Çü±¤ ´Ü¹éÁú·Î, ÇØ¾ç »ý¹°ÀÎ ÇØÆĸ®¿¡¼ À¯·¡ÇÑ °ÍÀÌ´Ù. GFP´Â ±× ÀÚ¡¦ |
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GFP ´Ü¹éÁú°ú ´Ü¹éÁú Á¤·®(Protein Measurements) / ¥°. Abstract ¥±. Introduction ¥². Materials and Methods ¥³. Results ¥´. Discussion ¥µ. References / ¥°. Abstract GFP(³ì»ö Çü±¤ ´Ü¹éÁú)´Â ÇØ¾ç »ý¹°ÀÎ ÇØÆĸ®¿¡¼ óÀ½ ¹ß°ßµÈ ´Ü¹éÁú·Î, µ¶Æ¯ÇÑ Çü±¤ Ư¼ºÀ¸·Î ÀÎÇØ »ý¹°ÇÐÀû ¿¬±¸¿¡¼ ³Î¸® »ç¿ëµÇ°í ÀÖ´Ù. GFP´Â ƯÁ¤ ÆÄÀåÀÇ ºûÀ» Èí¼öÇÑ ÈÄ ´Ù¸¥ ÆÄÀåÀÇ ºûÀ» ¹æÃ⡦ |
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UAS-GAL4 systemÀ» ÀÌ¿ëÇÑ ÃÊÆĸ®ÀÇ GFP ¹ßÇö ·¹Æ÷Æ® / 1. Object 2. Result 3. Discussion 1) °á°ú¿¹»ó 2) °á°úºÐ¼® 3) Ãß°¡Á¶»ç 4. Reference / 1. Object UAS-GAL4 ½Ã½ºÅÛÀ» ÀÌ¿ëÇÑ ÃÊÆĸ®ÀÇ GFP ¹ßÇö¿¡ °üÇÑ ¿¬±¸ÀÇ ÁÖ¿ä ¸ñÀûÀº ƯÁ¤ ¼¼Æ÷ À¯ÇüÀ̳ª Á¶Á÷¿¡¼ÀÇ À¯ÀüÀÚ ¹ßÇöÀ» Á¶ÀýÇÏ°í ½Ã°¢ÈÇÏ´Â µ¥ ÀÖ´Ù. ÀÌ ½Ã½ºÅÛÀº À¯ÀüÀÚ ¹ßÇöÀ» Á¤¹ÐÇÏ°Ô Á¦¾îÇÒ ¼ö ÀÖ´Â °·ÂÇÑ ¡¦ |
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