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ÆÄÁö ¹ÙÀÌ·¯½º °ËÁ¤ ½ÇÇè ·¹Æ÷Æ®, E.coli, bateriophage, lytic cycle, lysogenic cycle, plaque assay / 1. ½ÇÇè Á¦¸ñ 2. ½ÇÇè ¸ñÀû 3. ½ÇÇè ¿ø¸® 1) E.coli(Escherichia coli) 2) Phage virus 3) Lytic cycle, lysogenic cycle 4) Plaque assay 4. ½ÇÇè ±â±¸ ¹× Àç·á 5. ½ÇÇè ¹æ¹ý 6. Âü°í¹®Çå / 1. ½ÇÇè Á¦¸ñ ½ÇÇè Á¦¸ñÀº `E. coli¸¦ ´ë»óÀ¸·Î ÇÑ ¹ÚÅ׸®¿ÀÆÄÁöÀÇ ¶ó¡¦ |
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Glycine°ú LysineÀÇ maillard ¹ÝÀÀ ½ÇÇè / 1. ½ÇÇè Á¦¸ñ 2. ½ÇÇè ³¯Â¥ 3. ½ÇÇèÀÇ ¿äÁö(abstract) 4. ½ÇÇèÀÇ ¸ñÀû 5. ½ÇÇè ÀåÄ¡, Àç·á ¹× ½Ã¾à 6. ½ÇÇè ¹æ¹ý 7. ½ÇÇè °á°ú¿Í °íÂû 8. Âü°í¹®Çå / 1. ½ÇÇè Á¦¸ñ Glycine°ú LysineÀÇ Maillard ¹ÝÀÀ ½ÇÇè Maillard ¹ÝÀÀÀº ´Ü¹éÁú°ú ȯ¿ø´ç °£ÀÇ º¹ÀâÇÑ ÈÇÐ ¹ÝÀÀÀ¸·Î, ÁÖ·Î Á¶¸®¿Í °¡°ø °úÁ¤¿¡¼ ³ªÅ¸³ª´Â ±î¸Ä°í °í¼ÒÇÑ ¸ÀÀ» »ý¡¦ |
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LysosomeÀÇ ÃÖ±Ùµ¿Çâ¿¡ ´ëÇØ Á¶»çÇÑ ÀÚ·áÀÔ´Ï´Ù. LysosomeÀÇÃÖ±Ùµ¿Çâ / Æĺ긮º´Àº µå¹°°Ô ¹ß»ýÇϱ⠶§¹®¿¡ ÈçÈ÷ °í¾Æº´(orphan disease)À̶ó°í ºÒ¸®±âµµ ÇÑ ´Ù. ¸®¼ÒÁ» ÀúÀå ±â´É¿¡ ÀÌ»óÀÌ ¹ß»ýÇØ ¹ß»ýÇÏ´Â Áúº´À¸·Î´Â Æĺ긮º´ ¿Ü¿¡ ¶Ç ´Ù¸¥ ¿©¼¸ °¡Áö Áúº´ÀÌ ¾Ë·ÁÁ® Àִµ¥, À̵é Áúº´µµ Æĺ긮º´ Ä¡·á¹ý°ú µ¿ÀÏÇÑ ¹æ¹ýÀ¸·Î Ä¡·á°¡ °¡´ÉÇÏ´Ù°í ¿¬±¸ÁøÀº ¹àÇû´Ù. À̵é Áúº´ ¸ðµÎ°¡ ºñ½Á¡¦ |
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Lysosome ÀÌ»ó Áõ»ó¿¡ ´ëÇÑ ±ÛÀÔ´Ï´Ù. lysosome- / (1)ÇϳªÀÇ ¸®¼Ò¼û È¿¼ÒÀÇ °áÇÌ, (2) ¸®¼Ò¼Ø °ü·Ã °øÆ÷¿¡ ±âÁúÀÇ ÀÌ»ó ÃàÀûÀ¸·Î Á¤ÀÇµÇ°Ô ÇÏ¿´´Ù. ÀÌ·¯ÇÑ Á¤ÀÇ´Â º¯ÇüµÇ¾î, Çϳª³ª ±× ÀÌ»óÀÇ ¸®¼Ò¼Ø È¿¼Ò À¯ÀüÀÚ °áÇÔ¿¡ ÀÇÇÑ ¹ÂÄÚÁöÁúÁõ(mucolipidose)À̳ª, sulfatase °áÇÌÁõ±îÁö È®´ëµÇ¾ú´Ù. ¶ÇÇÑ ´õ¿í È®´ëµÇ¾î ¸®¼Ò¼Û ±â´É¿¡ ÇÊ¿äÇÑ ´Ü¹éÀÇ °áÇÌÁõ ¿¹¸¦ µé¾î spingolipid È°¼º ´Ü¡¦ |
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È¿¼Ò Áß ¶óÀ̼ÒÀÚÀÓÀÇ ÃֽŠÀÌ¿ëºÐ¾ß / È¿¼Ò±¹Á¦À§¿øȸ°¡ Á¤ÇÑ ¶óÀ̼ÒÀÚÀÓÀÇ EC numberÀº EC 3.2.1.17 ÀÌ´Ù. Æä´Ï½Ç¸° ¹ß°ßÀÚÀÎ FlemingÀÌ ¹ß°ßÇÑ Lysozyme (¶Ç´Â muramidase)Àº µ¿¹°¿¡ ÀÇÇØ »ý¼ºµÇ´Â Ç×±Õ¼º È¿¼ÒÀÌ´Ù. ±×¶÷¾ç¼º±Õ ¼¼Æ÷º®ÀÇ ÁÖ¼ººÐÀÎ ÆéƼµµ±Û¸®Ä(¹Â·¹ÀÎ)ÀÇ N-acetylmuramic acid °ú N-acetylglucosamine»çÀÌÀÇ ¥â(1-4)°áÇÕÀ» °¡¼öºÐÇؽÃÅ°´Â È¿¼ÒÀÌ´Ù. ÀÌ·¯ÇÑ °¡¼öºÐÇØ¡¦ |
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[ÀÚ¿¬°úÇÐ] À̿±³È¯ Chromatography¿¡ ÀÇÇÑ ´Þ°¿ ÈòÀÚ Lysozyme ºÐ¸® - ¹Ì¸®º¸±â¸¦ Âü°í ¹Ù¶ø´Ï´Ù. / À̿±³È¯ Chromatography¿¡ ÀÇÇÑ ´Þ°¿ ÈòÀÚ Lysozyme ºÐ¸® 1. ½ÇÇèÁ¦¸ñ : À̿±³È¯ Chromatography¿¡ ÀÇÇÑ ´Þ°¿ ÈòÀÚ Lysozyme ºÐ¸® 2. ½ÇÇèÀϽà : 2009. 10. 8 3. ½ÇÇè¸ñÀû : ¼¼Æ÷¿¡´Â ¼ö¸¹Àº Á¾·ùÀÇ ´Ü¹éÁúÀÌ ÇÔÀ¯µÇ¾î ÀÖÀ¸¸ç, À̵éÀÇ ¾Æ¹Ì³ë»ê Á¶¼ºÀ̳ª ¹è¿À» °áÁ¤ÇÏ°í ¼ºÁúÀ»¡¦ |
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1. Title Adherent Cell Lysis for Protein Extraction ¼¼Æ÷ »ý¹°ÇÐ ¹× »ýÈÇÐ ¿¬±¸¿¡¼ ´Ü¹éÁú ÃßÃâÀº Áß.. / 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference / 1. Title Adherent Cell Lysis for Protein Extraction ¼¼Æ÷ »ý¹°ÇÐ ¹× »ýÈÇÐ ¿¬±¸¿¡¼ ´Ü¹éÁú ÃßÃâÀº Áß¿äÇÑ °úÁ¤ÀÌ´Ù. ƯÈ÷, ºÎÂø ¼¼Æ÷¡¦ |
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Cell Transfection and Cell lysis ·¹Æ÷Æ® / 1. Background Cell Transfection 1-1 Cell transfection 1-2 HEK-293T cell 1-3 Epitope tagging 1-4 Transfection reagent Cell Lysis 1-a Cell lysis 1-b Lysis buffer 2. Purpose and Method Cell Transfection 2-1 Purpose 2-2 Method Cell Lysis 2-a Purpose 2-b Method 3. Result - 3-1 Cell picture and Cell lysis ¡¦ |
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/ 1. Title Adherent Cell Lysis for Protein ExtractionÀº ¼¼Æ÷ »ý¹°ÇÐ ¹× »ýÈÇÐ ºÐ¾ß¿¡¼ Áß¿äÇÑ ÁÖÁ¦ÀÌ´Ù. ¸¹Àº ¿¬±¸¿¡¼ ´Ü¹éÁúÀÇ ±â´É, ±¸Á¶ ¹× »óÈ£ÀÛ¿ëÀ» ÀÌÇØÇϱâ À§ÇØ ¼¼Æ÷ ³» ´Ü¹éÁúÀ» ÃßÃâÇÏ´Â °úÁ¤ÀÌ ÇʼöÀûÀÌ´Ù. ¼¼Æ÷´Â ´Ù¾çÇÑ ¹°Áú·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, ƯÁ¤ ´Ü¹éÁúÀ» ¿¬±¸Çϱâ À§Çؼ´Â ¼¼Æ÷¸·À» ¿øÈ°ÇÏ°Ô Æı«ÇÏ°í ³»ºÎÀÇ ´Ü¹éÁúÀ» È¿°úÀûÀ¸·Î ÃßÃâÇØ¾ß ÇÑ´Ù. ÀÌ´Â ¡¦ |
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Cell Transfection and Cell lysis ·¹Æ÷Æ® / 1. Background Cell Transfection 1-1 Cell transfection 1-2 HEK-293T cell 1-3 Epitope tagging 1-4 Transfection reagent Cell Lysis 1-a Cell lysis 1-b Lysis buffer 2. Purpose and Method Cell Transfection 2-1 Purpose 2-2 Method Cell Lysis 2-a Purpose 2-b Method 3. Result - 3-1 Cell picture and Cell lysis ¡¦ |
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