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�씠�삩援먰솚 Chromatography�뿉 �쓽�븳 �떖嫄� �씛�옄 Lysozyme 遺꾨━
1. �떎�뿕�젣紐� : �씠�삩援먰솚 Chromatography�뿉 �쓽�븳 �떖嫄� �씛�옄 Lysozyme 遺꾨━
2. �떎�뿕�씪�떆 : 2009. 10. 8
3. �떎�뿕紐⑹쟻 : �꽭�룷�뿉�뒗 �닔留롮�� 醫낅쪟�쓽 �떒諛깆쭏�씠 �븿�쑀�릺�뼱 �엳�쑝硫�, �씠�뱾�쓽 �븘誘몃끂�궛 議곗꽦�씠�굹 諛곗뿴�쓣 寃곗젙�븯怨� �꽦吏덉쓣 洹쒕챸�븯湲� �쐞�빐�꽌�뒗 癒쇱�� �떒諛깆쭏�쓣 異붿텧�빐 �궡�빞 �빀�땲�떎. �씠�삩援먰솚 Chromatography瑜� 鍮꾨’�븳 �뿬�윭 �젙�젣踰뺤뿉 �쓽�빐 �닚�닔�븳 �떒諛깆쭏�쓣 �뼸�뒗 寃껋�� �떒諛깆쭏 �뿰援ъ쓽 媛��옣 湲곕낯�씠硫� �븘�닔�쟻�씤 怨쇱젙�엯�땲�떎.
4. �옱猷� 諛� 湲곌뎄 : (1) 湲곌뎄
�몺 �떖嫄�
�몼 鍮꾩빱
�몾 嫄곗쫰
�몿 �뵾�렖(10�럷, 200�럶)
�뫀 Tube(5媛�)
�뫁 二쇱궗湲�(Chromatography Column)
�뫂 �뙆�씪�븘由�
�뫃 �쑀由ъ넑
�뫅 吏�吏����
�뫆 �뒪�깲�뱶
(2)�떆�빟
�몺 Sephadex(CM-Sephadex C-25; Pharmacia)
�몼 Tris-EDTA buffer 0.05M(0.05M tris , 0.004 EDTA, 0.05M , 8.2)
�몾 �깂�궛 buffer 0.2M(0.2M 800�럷, 0.2M , 10.5)
5. �떎�뿕�씠濡� 諛� �썝由�
�뾾 湲곕낯媛쒕뀗
�뼴 Lysozyme
Lysozyme��� �꽭洹좎쓽 �꽭�룷踰쎌쓣 �씠猷④퀬 �엳�뒗 �떎�떦瑜섏쓽 援ъ꽦�꽦遺꾩씤 N-acetylmuarmic acid(NAM)��� N-acetylglucosamine(NAG) �궗�씠�쓽 棺-쨌1,4 �뿰寃곗쓣 媛��닔遺꾪빐�븯�뒗 �슚�냼�엯�땲�떎.
�씠誘� �닚�닔�븯寃� 遺꾨━�릺�뼱 �븘誘몃끂�궛 �꽌�뿴�씠 寃곗젙�릺�뿀�쑝硫� �엯泥닿뎄議곌�� 媛��옣 癒쇱�� �븣�젮吏� �떒諛깆쭏�엯�땲�떎. Lysozyme��� �떎瑜� �떒諛깆쭏�뿉 鍮꾪빐 �벑���(�깮�왂)
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