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ÀçÁ¶ÇÕ DNA¸¦ ¼÷ÁÖ ¼¼Æ÷(´ëÀå±Õ)¿¡ »ðÀÔ(transformation)ÇÏ¿© ±×°ÍÀ» ´Ù·® ¹ßÇöÇÏ´Â ½ÇÇèÀÇ ¸®Æ÷Æ®ÀÔ´Ï´Ù. colonyoverexpression / 3.Methods ¨ç transformationµÈ E coli¸¦ kanamycin(100 ug/ml)ÀÌ Ã·°¡µÈ LB¹èÁö¿¡ Á¢Á¾ ¨è ¼·¾¾ 37µµ¿¡¼¡¡ cultureÇÏ¿© Èí±¤µµ 600nm¿¡¼¡¡OD°ªÀÌ 0.6ÀÌ µÇµµ·Ï ¹è¾ç ¨é IPTG¸¦ ³óµµ°¡ 1mMÀÌ µÇµµ·Ï ÷°¡, ¹ßÇö À¯µµ 3½Ã°£ Induction½ÃŲ °Í (Á¶±³µéÀÌ ¡¦ |
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CFU ¹Ì»ý¹° ½ÇÇ躸°í¼, Colony Forming Unit / 1. ÃÊ·Ð (Abstract) 2. ¼·Ð (Introduction) 3. ½ÇÇè Àç·á ¹× ¹æ¹ý (Experimental Materials and Methods) 4. °á·Ð (Result) 5. °íÂû (Discussion) 6. Âü°í ¹®Çå (References) / 1. ÃÊ·Ð (Abstract) CFU(Colony Forming Unit)´Â ¹Ì»ý¹°ÀÇ ¼ö¸¦ ÃøÁ¤ÇÏ´Â Áß¿äÇÑ ÁöÇ¥·Î, ¹Ì»ý¹° ¹è¾ç ½ÇÇè¿¡¼ ƯÁ¤ ȯ°æ Á¶°Ç¿¡¼ »ýÀ°ÇÒ ¼ö ÀÖ´Â ¹Ì¡¦ |
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CFU ¹Ì»ý¹° ½ÇÇ躸°í¼, Colony Forming Unit / 1. ÃÊ·Ð (Abstract) 2. ¼·Ð (Introduction) 3. ½ÇÇè Àç·á ¹× ¹æ¹ý (Experimental Materials and Methods) 4. °á·Ð (Result) 5. °íÂû (Discussion) 6. Âü°í ¹®Çå (References) / 1. ÃÊ·Ð (Abstract) Colony Forming Unit, CFU´Â ¹Ì»ý¹°Çп¡¼ ¼¼±ÕÀ̳ª °õÆÎÀ̸¦ Æ÷ÇÔÇÑ ¹Ì»ý¹°ÀÌ ´Üµ¶ ¶Ç´Â ÁýÇÕü·Î ÀÚ»ýÇÒ ¼ö ÀÖ´Â ´É·ÂÀ» °¡Áø ¼¼¡¦ |
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Colony formation assay A£«·¹Æ÷Æ® / 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference / 1. Title Colony formation assay´Â ¼¼Æ÷ÀÇ ¼ºÀå ´É·Â°ú »ýÁ¸ ´É·ÂÀ» Æò°¡Çϱâ À§ÇÑ ½ÇÇè ±â¹ýÀÌ´Ù. ÀÌ ¹æ¹ýÀº ¼¼Æ÷°¡ ´ÜÀÏ ¼¼Æ÷·ÎºÎÅÍ µ¶¸³ÀûÀÎ Áý¶ôÀ» Çü¼ºÇÏ´Â ´É·ÂÀ» ±â¹ÝÀ¸·Î Çϸç, ¾Ï ¿¬±¸, ¾à¹° ¹ÝÀÀ¼º Æò°¡¡¦ |
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colony pcr °á°ú·¹Æ÷Æ® / 1. ½ÇÇè°á°ú 2. ÅäÀÇ ¹× °íÂû / 1. ½ÇÇè°á°ú ÄÝ·Î´Ï PCRÀÇ ½ÇÇè °á°ú¸¦ ÅëÇØ È®ÀÎµÈ ³»¿ëÀº ¿©·¯ °¡Áö Áß¿äÇÑ Á¤º¸¸¦ Á¦°øÇÑ´Ù. ½ÇÇè¿¡¼ »ç¿ëµÈ »ùÇõéÀº ƯÁ¤ À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÏ°íÀÚ ÇÏ´Â Çö󽺹̵尡 Ŭ·Î´×µÈ ¹ÚÅ׸®¾ÆÀÇ ÄݷδϿ¡¼ À¯·¡ÇÑ °ÍÀÌ´Ù. PCRÀ» ÅëÇØ ¾òÀº °á°ú´Â °¢ ÄݷδÏÀÇ À¯ÀüÀÚ Å¬·Î´× ¼º°ø ¿©ºÎ¸¦ ÆÇ´ÜÇÏ´Â Áß¿äÇÑ ±âÁØÀÌ µÈ´Ù. ÃÖÁ¾ °á¡¦ |
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½Ä¹°ºÐÀÚ»ý¹°ÇнÇÇè Colony PCR °á°úº¸°í¼ / 1. ½ÇÇèÁ¦¸ñ 2. ½ÇÇèÀÏÀÚ 3. ½ÇÇè°³¿ä 4. ½ÇÇèÀÌ·Ð 5. Materials and methods 6. ½ÇÇè°á°ú 7. Discussion 8. References / 1. ½ÇÇèÁ¦¸ñ ½Ä¹°ºÐÀÚ»ý¹°ÇнÇÇè¿¡¼ Colony PCR¸¦ ¼öÇàÇÑ ¿¬±¸ÀÇ ÁÖÁ¦¸¦ ´Ù·ç´Â °Í¿¡´Â À¯ÀüÀÚ ºÐ¼® ¹× ¹ßÇö ¿¬±¸¿¡¼ Áß¿äÇÑ ´Ü°è°¡ Æ÷ÇԵȴÙ. Colony PCR´Â ƯÁ¤ À¯ÀüÀÚ¸¦ °¡Áø ¹Ì»ý¹° Áý¶ôÀ» ½Å¼ÓÈ÷ ½Ä¡¦ |
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A£«½ÄÇ°¹Ì»ý¹°ÇнÇÇè ·¹Æ÷Æ®(Isolation of single colony) / 1. Abstract 2. Introduction 3. Materials&Method 4. Result&Discussion 1) ½ÇÇè °á°ú ºñ±³ ¹× Çؼ® 2) StreakingÀÌ ±ò²ûÇÏ°Ô µÇÁö ¾ÊÀº ÀÌÀ¯ & Streaking ÀßÇÏ´Â ¹æ¹ý 3) Pediococcus acidilactici ±ÕÀÇ È°¿ë ¹× streaking °úÁ¤ ºñ±³ 5. Homework 6. Reference / 1. Abstract ¹è¾ç ÈÄ ¹Ì»ý¹°ÀÇ ¼ø¼ö ºÐ¸®¿Í¡¦ |
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T-vector¿Í insert DNA ºñÀ²À» ´Þ¸®ÇÏ¿© TA cloningÀ» ÅëÇØ ¾ò¾îÁø colony ºñ±³ ºÐ¼® / 1. Subject 2. Object 3. Introduction 4. Material & Method 5. Result 6. Discussion / 1. Subject TA Ŭ·Î´×(TA cloning)Àº ƯÁ¤ÇÑ Á¦ÇÑ È¿¼Ò ¾øÀÌ DNA ´ÜÆíÀ» Ŭ·Î´×ÇÏ´Â ÇÑ ¹æ¹ýÀ¸·Î, ÁÖ·Î T-vector¸¦ ÀÌ¿ëÇÏ¿© ¿øÇÏ´Â DNA Á¶°¢À» È¿À²ÀûÀ¸·Î Ŭ·Î´×ÇÏ´Â µ¥ »ç¿ëµÈ´Ù. T-vector´Â ÀÏ¡¦ |
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Transformation , Colony PCR »ý¹°°øÇнÇÇè º¸°í¼ / ¥°. 11ÁÖÂ÷ ¿¹ºñº¸°í¼ (Transformation) 1. TransformationÀ» ¼öÇàÇÏ´Â ¸ñÀûÀÌ ¹«¾ùÀÎÁö ¼³¸íÇϽÿÀ. 2. Competent cellÀÇ °³³ä°ú Ư¡¿¡ ´ëÇØ ¼³¸íÇϽÿÀ. 3. Transformation ½ÇÇè ÁøÇà ½Ã ÁÖÀÇ»çÇ׿¡ ´ëÇÏ¿© ¼¼ °¡Áö ÀÌ»ó ³íÇϽÿÀ. ¥±. 12ÁÖÂ÷ ¿¹ºñº¸°í¼ (Colony PCR) 1.Colony PCRÀ» ¼öÇàÇÏ´Â °úÁ¤¿¡¼ colony¸¦ pickÇÑ¡¦ |
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[¹Ì»ý¹°½ÇÇè]Colony Isolation / 1. ½ÇÇè ¸ñÀû 2. ½ÇÇè ÀÌ·Ð ¹× ¿ø¸® 3. ½ÇÇè ±â±¸ ¹× ½Ã¾à 4. ½ÇÇè ¹æ¹ý 5. ½ÇÇè °á°ú 6. ÅäÀÇ »çÇ× 7. Âü°í ¹®Çå / 1. ½ÇÇè ¸ñÀû ÄÝ·Î´Ï ¾ÆÀַ̼¹À̼ÇÀº ¹Ì»ý¹°Çп¡¼ Áß¿äÇÑ ±â¹ý Áß Çϳª·Î, ´Ù¾çÇÑ ¹Ì»ý¹° Áý´Ü¿¡¼ ƯÁ¤ÇÑ ¹Ì»ý¹° °³Ã¼¸¦ ºÐ¸®ÇÏ¿© ¼ø¼ö ¹è¾çÇÏ´Â °úÁ¤ÀÌ´Ù. ÀÌ ½ÇÇèÀÇ ¸ñÀûÀº ƯÁ¤ ¹Ì»ý¹° Á¾À» ´Üµ¶À¸·Î ºÐ¸®ÇÔÀ¸·Î½á ÇØ´ç ¹Ì¡¦ |
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