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À¯±âÈÇнÇÇè Triphenylcarbinol- Addition of a Grignard Reagent to a Ketone °á°úº¸°í¼ / 1. Subject 2. Date&Section 3. Object 4. Procedure 5. Result 6. Discussion / 1. Subject À¯±âÈÇÐ ½ÇÇèÀÇ ÁÖÁ¦´Â ±×¸®³Ä¸£ ½Ã¾àÀ» ÀÌ¿ëÇÑ ÄÉÅæÀÇ ¹ÝÀÀÀ» ÅëÇØ Æ®¸®Æä´ÒÄ«¸£ºñ³îÀ» ÇÕ¼ºÇÏ´Â °ÍÀÌ´Ù. ±×¸®³Ä¸£ ½Ã¾àÀº À¯±âÈÇп¡¼ Áß¿äÇÑ ¿ªÇÒÀ» ÇÏ´Â ¹ÝÀÀ¼º ÈÇÕ¹°·Î, ÁÖ·Î ¾Ëų ¡¦ |
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¹ÙÀÌ·¯½ºÇÐ ½ÇÇè °á°úº¸°í¼ Trizol reagent method for RNA extraction / 1. Title 2. Objective 3. Principle 4. Method 5. Result 6. Discussion 7. References / 1. Title Trizol reagent method for RNA extractionÀº ¼¼Æ÷ ¶Ç´Â Á¶Á÷¿¡¼ RNA¸¦ È¿°úÀûÀ¸·Î ºÐ¸® ¹× Á¤Á¦Çϱâ À§ÇÑ ³Î¸® »ç¿ëµÇ´Â ¹æ¹ýÀ¸·Î, ÀÌ ¹æ¹ýÀº 1987³â Chomczynski¿Í Sacchi¿¡ ÀÇÇØ Ã³À½ °³¹ßµÇ¾ú´Ù.¡¦ |
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À¯±âÈÇнÇÇè Triphenylcarbinol- Addition of a Grignard Reagent to a Ketone °á°úº¸°í¼ / 1. Subject 2. Date&Section 3. Object 4. Procedure 5. Result 6. Discussion / 1. Subject À¯±âÈÇнÇÇèÀÇ ÁÖÁ¦´Â Æ®¸®Æä´ÒÄ«¸£ºñ³îÀÇ ÇÕ¼ºÀ» À§ÇÑ ±×¸®³Ä¸£ ½Ã¾àÀÇ ÄÉÅæ¿¡ ´ëÇÑ Ã·°¡ ¹ÝÀÀÀÌ´Ù. ÀÌ ½ÇÇèÀÇ ¸ñÀûÀº ±×¸®³Ä¸£ ½Ã¾àÀÌ ÄÉÅæ°ú ¹ÝÀÀÇÏ¿© ¾ËÄÚ¿ÃÀ» »ý¼ºÇÏ´Â °úÁ¤°ú ±×·Î ÀΡ¦ |
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À¯±âÈÇнÇÇè, grignard reagent¸¦ ÀÌ¿ëÇÑ ¹ÝÀÀ / 1. Purpose 2. Theory 1) Preparation of Grignard reagent 2) Grignard reaction and mechanism 3) Grignard reaction with carbonyl Compounds and its mechanism 4) Limitation of using Grignard reagent 3. Reagents & Apparatus 1) Reagent 2) Apparatus 4. Physical properties of reagent 5. Data&Results 6. Discuss¡¦ |
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¹ÙÀÌ·¯½ºÇÐ Trizol reagent method for RNA extraction ½ÇÇè¿¡ ´ëÇÑ °á°ú º¸°í¼. ÀÌ ½ÇÇèÀº RT-PCRÀ» ÇϱâÀ§Çؼ Trizol reagent method¸¦ ÀÌ¿ëÇØ poliovirusÀÇ RNA¸¦ ºÐ¸®ÇÏ°í Á¤Á¦ÇÏ´Â ½ÇÇèÀ¸·Î º»¹®Àº ½ÇÇè ¸ñÇ¥, ¿ø¸®, °á°ú»çÁø, ½ÇÇè ¹æ¹ý ¼³¸í, RNA ±¸Á¶ÀÇ ºÒ¾ÈÁ¤¼º, RNas ¿À¿° ¹× Á¦°ÅÆ÷ÇÔ / 1. Title 2. Objective 3. Principle 4. Method 5. Result 6. Discussion 7. Re¡¦ |
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A£« ¹ÞÀº À¯±âÈÇнÇÇè 2 Exp 7 Triphenylcarbinol Addition of a Grignard Reagent to a Ketone (½ÇÇè¹æ¹ý, ÇÁ¸®·¦, ·¦¸®Æ÷Æ® ¸ðÀ½) / 1. ½ÇÇè¹æ¹ý 2. ÇÁ¸®·¦ 3. ·¦¸®Æ÷Æ® / 1. ½ÇÇè¹æ¹ý ½ÇÇèÀº Æ®¸®Æä´ÒÄ«¸£ºó¿Ã(triphenylcarbinol)À» ÇÕ¼ºÇϱâ À§ÇÑ ±×¸®³Ä¸£ ½Ã¾àÀÇ ÄÉÅæ¿¡ ´ëÇÑ Ã·°¡ ¹ÝÀÀÀ» ¿¬±¸ÇÏ´Â °ÍÀÌ´Ù. ÀÌ ½ÇÇè¿¡¼ »ç¿ëµÇ´Â ÁÖ¿ä ÈÇÕ¹°ÀÎ ¾Æ¼¼ÅæÀº ¹ÝÀÀÀÇ ±âÃÊ·Î ÀÛ¿ëÇÏ¡¦ |
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A£« ¹ÞÀº À¯±âÈÇнÇÇè 2 Exp 7 Triphenylcarbinol Addition of a Grignard Reagent to a Ketone (½ÇÇè¹æ¹ý, ÇÁ¸®·¦, ·¦¸®Æ÷Æ® ¸ðÀ½) / 1. ½ÇÇè¹æ¹ý 2. ÇÁ¸®·¦ 3. ·¦¸®Æ÷Æ® / 1. ½ÇÇè¹æ¹ý À̹ø ½ÇÇè¿¡¼´Â Grignard ½Ã¾àÀ» »ç¿ëÇÏ¿© ÄÉÅæ°ú ¹ÝÀÀ½ÃÄÑ triphenylcarbinolÀ» ÇÕ¼ºÇÏ´Â °úÁ¤¿¡ ´ëÇØ ´Ù·é´Ù. ½ÇÇè ½ÃÀÛ Àü¿¡ ÇÊ¿äÇÑ Àåºñ¿Í ÈÇй°ÁúÀ» ÁغñÇØ¾ß ÇÑ´Ù. ÇÊ¿äÇÑ Àåºñ·Î´Â ±Ù¡¦ |
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