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Ãʵ½Ç¿µ¾îÀÇ ¸ðµç Ç¥ÇöÀ» Á¤¸®ÇسõÀº ÀÚ·áÀÌ´Ù. ClassroomTeachingEnglish / Greetings (Àλç) Checking Attendance(Ãâ¼® Á¡°Ë) Physical Conditions in the Classroom(±³½Ç ȯ°æ Á¶¼º) Seating (ÀÚ¸® ¹è¿Çϱâ) Review & Today`s Lesson (º¹½À°ú ¿À´ÃÀÇ ¼ö¾÷) Handouts (À¯Àι°) Looking for Pages (ÆäÀÌÁö ã±â) Exercises (¿¬½À ¹®Á¦) Introducing Different Stages of the Lesson ¡¦ |
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English semantics - 5.9 symmetry and reciprocity , 5.10 expressions of quantity / English semantics - 5.9 symmetry and reciprocity & 5.10 expressions of quantity ¸ñÂ÷ 5.9 symmetry and reciprocity 5.10 expressions of quantity 5.9 symmetry and reciprocity ¿µ¾îÀÇ Àǹ̷п¡¼ ´ëĪ¼º°ú »óÈ£ÀÛ¿ëÀº Áß¿äÇÑ °³³äÀÌ´Ù. ´ëĪ¼ºÀº ÀǹÌÀÇ Çü¼º°ú ÀÌÇØ¿¡¼ ´ëĪÀûÀÎ °ü°è¸¦¡¦ |
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¡¥ns by colony PCR 8. stage8- Expression of fusion gene by IPTG indauction 9. stage9- Identification of Recombinant DNA 10. stage10- Purification of fusion protein by metal affinity chromatography / 1. stage1- Cell culture Ferritin fusion proteinÀÇ È¿À²ÀûÀÎ ¹ßÇöÀ» À§ÇØ ¼¼Æ÷ ¹è¾ç °úÁ¤Àº ÇʼöÀûÀÌ´Ù. º» ¿¬±¸¿¡¼´Â HEK293 ¼¼Æ÷ÁÖ¸¦ »ç¿ëÇÏ¿© ferritin À¶ÇÕ ´Ü¡¦ |
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gene expression / 1. Subject 2. Object 3. Introduction 4. Materials & Method 5. Result 6. Discussion 7. Reference / 1. Subject À¯ÀüÀÚ ¹ßÇö(gene expression)Àº »ý¸íüÀÇ ¼¼Æ÷°¡ ÇÑ À¯ÀüÀÚ¿¡¼ Á¤º¸¸¦ ÀÐ¾î ´Ü¹éÁúÀ» ÇÕ¼ºÇÏ´Â °úÁ¤À» ÀǹÌÇÑ´Ù. ÀÌ´Â »ý¸íü°¡ ±âÃÊÀûÀÎ ±â´ÉÀ» ¼öÇàÇÏ°í, ´Ù¾çÇÑ »ý¸®Àû °úÁ¤À» Á¶ÀýÇÏ´Â µ¥ ÇÙ½ÉÀûÀÎ ¿ªÇÒÀ» ÇÑ´Ù. À¯ÀüÀÚ´Â DNA·Î ±¸¼ºµÇ¡¦ |
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[°á°ú·¹Æ÷Æ®] Liquid culture , Expression / 1. Abstract 2. Experiment 3. Result 4. Discussion 5. Reference / 1. Abstract ¾×ü ¹è¾ç ¹× ¹ßÇö¿¡ °üÇÑ ¿¬±¸´Â »ý¸í°úÇÐ ¹× »ý¹°°øÇÐ ºÐ¾ß¿¡¼ Áß¿äÇÑ ¿ªÇÒÀ» Â÷ÁöÇÏ°í ÀÖ´Ù. ÀÌ º¸°í¼´Â ¾×ü ¹è¾ç ½Ã½ºÅÛÀÇ ±âº» ¿ø¸®¿Í ±×¿¡ µû¸¥ ´Ü¹éÁú ¹ßÇö ¸ÞÄ¿´ÏÁòÀ» ´Ù·ç°í, ´Ù¾çÇÑ ÀÀ¿ë »ç·Ê¸¦ ÅëÇØ ÀÌµé ½Ã½ºÅÛÀÇ È¿À²¼ºÀ» Ž±¸ÇÑ´Ù.¡¦ |
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[º¸°Çȯ°æ¹Ì»ý¹°ÇнÇÇè]Heterologous expression of the transformed gene / 1. ½ÇÇè ¸ñÀû 2. ½ÇÇè ÀÌ·Ð ¹× ¿ø¸® 3. ½ÇÇè ±â±¸ ¹× ½Ã¾à 4. ½ÇÇè ¹æ¹ý 5. ½ÇÇè °á°ú / 1. ½ÇÇè ¸ñÀû Heterologous expression of the transformed gene¿¡ °üÇÑ ½ÇÇèÀÇ ¸ñÀûÀº ƯÁ¤ À¯ÀüÀÚ¸¦ Ÿ »ý¹°Ã¼¿¡¼ ¹ßÇö½ÃÄÑ ±× ±â´É°ú Ư¼ºÀ» ¿¬±¸ÇÏ´Â °ÍÀÌ´Ù. ÀÌ ½ÇÇèÀº À¯ÀüÀÚ°¡ ¿øÇÏ´Â ´Ü¹éÁú·Î ÀüȯµÇ¾î ¡¦ |
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IPTG induction (expression)°ü·Ã ·¹Æ÷Æ® (A£«¹ÞÀºÀÚ·á) / 1. IPTG induction (expression) 2. IPTG induction ½Ã expressionÀÌ µÇÁö ¾Ê´Â °æ¿ì 3. Âü°í³»¿ë / 1. IPTG induction (expression) IPTG À¯µµ ¹× ¹ßÇö¿¡ ´ëÇØ ¼³¸íÇÏ°Ú´Ù. IPTG´Â À̼ÒÇÁ·ÎÇÊ-¥â-D-Ƽ¿À°¥¶ôÅäÇǶó³ë»çÀ̵å(Isopropyl ¥â-D-1-thiogalactopyranoside)ÀÇ ¾àÀÚ·Î, ÁÖ·Î ´ëÀå±ÕÀ» Æ÷ÇÔÇÑ ¹Ì»ý¹°¿¡¼ ÀçÁ¶ÇÕ ´Ü¡¦ |
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