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Membrane blocking ÇÊ¿äÇÑ ÀÌÀ¯, blockingÀ» ÇÒ ¶§ »ç¿ëÇÏ´Â ½Ã¾à, primary antibody¿Í secondary antibody·Î ±¸ºÐÇÏ´Â ÀÌÀ¯

Membrane blocking ÇÊ¿äÇÑ ÀÌÀ¯, blockingÀ» ÇÒ ¶§ »ç¿ëÇÏ´Â ½Ã¾à, primary antibody¿Í secondary antibody·Î ±¸ºÐÇÏ´Â ÀÌÀ¯

1. Membrane blockingÀÌ ÇÊ¿äÇÑ ÀÌÀ¯ Membrane blockingÀÌ ÇÊ¿äÇÑ ÀÌÀ¯´Â ´Ù¾çÇÑ ½ÇÇè¿¡¼­ ºñƯÀÌÀûÀÎ °áÇÕÀ» ¿¹¹æÇÏ°í.. / 1. Membrane blockingÀÌ ÇÊ¿äÇÑ ÀÌÀ¯ 2. blockingÀ» ¼öÇàÇϱâ À§ÇØ »ç¿ëÇÏ´Â ½Ã¾à 3. Western blot Ç×ü ¹ÝÀÀ ½Ã primary antibody¿Í secondary antibody·Î ±¸ºÐÇÏ¿© »ç¿ëÇÏ´Â ÀÌÀ¯´Â ¹«¾ùÀΰ¡ 4. Âü°í¹®Çå / 1. Membrane blockingÀÌ ÇÊ¿äÇÑ ÀÌÀ¯ Membran¡¦
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[ÀÌÈ­¿©´ë »ý¸í°úÇнÇÇè3 ºÐ¹Ý1µî A£« ·¹Æ÷Æ®] Detection of Antibody

[ÀÌÈ­¿©´ë »ý¸í°úÇнÇÇè3 ºÐ¹Ý1µî A£« ·¹Æ÷Æ®] Detection of Antibody

I. Objective Detection of Antibody ½ÇÇèÀÇ ¸ñÀûÀº ƯÁ¤ Ç×ü¸¦ È¿°úÀûÀ¸·Î °ËÃâÇÏ°í Á¤·®È­ÇÏ¿© ¸é¿ª ¹ÝÀÀÀ» ÀÌÇØÇÏ°í .. / I. Objective II. Assay Principle 1. Antibody 2. B Cell Maturation and Humoral Response 3. TD/TI Antigen 4. ELISA (Enzyme-Linked Immunosorbent Assay) III. Materials IV. Assay Protocol 1. Cell Culturing 2. Supernatant Harvesting ¡¦
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antibody binding, blocking, detection¿¡ °üÇÑ ·¹Æ÷Æ® (µ¶¼ºÇÐ)

antibody binding, blocking, detection¿¡ °üÇÑ ·¹Æ÷Æ® (µ¶¼ºÇÐ)

1. ½ÇÇè ¸ñÀû ½ÇÇè ¸ñÀûÀº Ç×üÀÇ °áÇÕ, Â÷´Ü ¹× °ËÃâ ¸ÞÄ¿´ÏÁòÀ» ÀÌÇØÇÏ°í À̸¦ ÅëÇØ µ¶¼ºÇÐÀû ºÐ¼®ÀÇ Á¤È®¼º°ú ½Å·Ú¼ºÀ» ³ôÀÌ´Â µ¥ ÀÖ´Ù. Ç×.. / 1. ½ÇÇè ¸ñÀû 2. ½ÇÇè°úÁ¤ 3. °á°ú 4. Reference / 1. ½ÇÇè ¸ñÀû ½ÇÇè ¸ñÀûÀº Ç×üÀÇ °áÇÕ, Â÷´Ü ¹× °ËÃâ ¸ÞÄ¿´ÏÁòÀ» ÀÌÇØÇÏ°í À̸¦ ÅëÇØ µ¶¼ºÇÐÀû ºÐ¼®ÀÇ Á¤È®¼º°ú ½Å·Ú¼ºÀ» ³ôÀÌ´Â µ¥ ÀÖ´Ù. Ç×ü´Â ¸é¿ª°èÀÇ Áß¿äÇÑ ±¸¼º ¿ä¼Ò·Î,¡¦
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[¾à´ë ½Ç½À ·¹Æ÷Æ®] ¸é¿ªÇÐ ½Ç½À ·¹Æ÷Æ® Hybridoma cell , antibody purification

[¾à´ë ½Ç½À ·¹Æ÷Æ®] ¸é¿ªÇÐ ½Ç½À ·¹Æ÷Æ® Hybridoma cell , antibody purification

[¾à´ë ½Ç½À ·¹Æ÷Æ®] ¸é¿ªÇÐ ½Ç½À ·¹Æ÷Æ® Hybridoma cell , antibody purification / 1. introduction 2. Protocol 3. Result 4. Discussion 5. Assignment 6. Pre-study 7. Reference / 1. introduction ¸é¿ªÇÐÀº »ýüÀÇ ¸é¿ª ½Ã½ºÅÛÀ» ¿¬±¸ÇÏ´Â Çй®À¸·Î, ÀÌ ½Ã½ºÅÛÀÇ Á¤»óÀûÀÎ ±â´É°ú ¸é¿ª °áÇÌ, ÀÚ°¡¸é¿ª Áúȯ, ¾Ë·¹¸£±â, °¨¿° Áúȯ µî ´Ù¾çÇÑ ¸é¿ª °ü·Ã ÁúȯÀ» ÀÌÇØÇÏ°í Ä¡¡¦
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[ÀÌÈ­¿©´ë »ý¸í°úÇнÇÇè3 ºÐ¹Ý1µî A£« ·¹Æ÷Æ®] Detection of Antibody

[ÀÌÈ­¿©´ë »ý¸í°úÇнÇÇè3 ºÐ¹Ý1µî A£« ·¹Æ÷Æ®] Detection of Antibody

[ÀÌÈ­¿©´ë »ý¸í°úÇнÇÇè3 ºÐ¹Ý1µî A£« ·¹Æ÷Æ®] Detection of Antibody / I. Objective II. Assay Principle 1. Antibody 2. B Cell Maturation and Humoral Response 3. TD/TI Antigen 4. ELISA (Enzyme-Linked Immunosorbent Assay) III. Materials IV. Assay Protocol 1. Cell Culturing 2. Supernatant Harvesting 3. ELISA V. Result & Observation 1. Result 2. D¡¦
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[¸é¿ªÇнǽÀ] Hybridoma cell and antibody purification

[¸é¿ªÇнǽÀ] Hybridoma cell and antibody purification

[¸é¿ªÇнǽÀ] Hybridoma cell and antibody purification / 1. Introduction 1) ´ÜÀÏŬ·ÐÇ×ü 2) HAT ¹èÁö 3) PC61 4) Resin 2. Protocol 1) ¸¶¿ì½º ¸é¿ª ȹµæ 2) ¼¼Æ÷ À¶ÇÕ 3) Ç×ü ¼öÁý 4) Ç×ü ºÐ¸® ¹× Á¤Á¦ 3. Results 4. Discussion 5. Assignments Monoclonal antibody, polyclonal antibody°¡ È°¿ëµÇ´Â ºÐ¾ß 1) Immunoassay 2) Treatment 6. References ¡¦
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Hybridoma ¼¼Æ÷ ¹è¾ç ¹× ¹è¾ç¾× ¼öÁý (£« monoclonal antibody Ä¡·áÁ¦)

Hybridoma ¼¼Æ÷ ¹è¾ç ¹× ¹è¾ç¾× ¼öÁý (£« monoclonal antibody Ä¡·áÁ¦)

Hybridoma ¼¼Æ÷ ¹è¾ç ¹× ¹è¾ç¾× ¼öÁý (£« monoclonal antibody Ä¡·áÁ¦) / ¥°. Á¦¸ñ ¹× ½ÇÇ賯¥ ¥±. ½ÇÇè¸ñÀû ¥². ¹è°æÁö½Ä ¥³. ½ÇÇè °úÁ¤ ¥´. °á°ú ¥µ. °íÂû ¥¶. Âü°í¹®Çå / ¥°. Á¦¸ñ ¹× ½ÇÇ賯¥ Hybridoma ¼¼Æ÷ ¹è¾ç ¹× ¹è¾ç¾× ¼öÁý ½ÇÇèÀº Çö´ë »ý¸í°úÇÐ ºÐ¾ß¿¡¼­ Áß¿äÇÑ °úÁ¤À¸·Î, ƯÈ÷ ´ÜŬ·Ð Ç×ü »ý»ê¿¡ ÀÖ¾î ÇʼöÀûÀÎ ±â¼úÀÌ´Ù. ´ÜŬ·Ð Ç×ü´Â ƯÁ¤ Ç׿ø¿¡ ´ëÇØ °íµµ¡¦
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[A£« ¸®Æ÷Æ®] Western blot 2 (Antibody , Detection(ECL

[A£« ¸®Æ÷Æ®] Western blot 2 (Antibody , Detection(ECL

[A£« ¸®Æ÷Æ®] Western blot 2 (Antibody , Detection(ECL / 1. Date 2. Title 3. Purpose 4. Materials 5. Method °¡. Antibody (Ç׿ø-Ç×ü ¹ÝÀÀ) ³ª. Detection 6. Result °¡. chemi-docÀ» ÀÌ¿ëÇÏ¿© ÂïÀº protein marker¿Í sampleÀÇ °á°ú »çÁø 7. Discussion °¡. Ç׿ø-Ç×ü ¹ÝÀÀ (Antigen-Antibody reaction) ³ª. DetectionÀÇ ¿ø¸® 8. Remark 9. Reference / 1.¡¦
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[¾à´ë ½Ç½À ·¹Æ÷Æ®] ¸é¿ªÇÐ ½Ç½À ·¹Æ÷Æ® Hybridoma cell , antibody purification

[¾à´ë ½Ç½À ·¹Æ÷Æ®] ¸é¿ªÇÐ ½Ç½À ·¹Æ÷Æ® Hybridoma cell , antibody purification

[¾à´ë ½Ç½À ·¹Æ÷Æ®] ¸é¿ªÇÐ ½Ç½À ·¹Æ÷Æ® Hybridoma cell , antibody purification / 1. introduction 2. Protocol 3. Result 4. Discussion 5. Assignment 6. Pre-study 7. Reference / 1. introduction ¸é¿ªÇÐ ºÐ¾ß´Â »ý¹°Ã¼ÀÇ ¸é¿ª ¹ÝÀÀÀ» ÀÌÇØÇÏ°í À̸¦ È°¿ëÇÏ¿© Áúº´ÀÇ ¿¹¹æ ¹× Ä¡·á¿¡ ±â¿©ÇÏ´Â Áß¿äÇÑ Çй® ºÐ¾ßÀÌ´Ù. ƯÈ÷, Ç×ü´Â ¸é¿ª°è¿¡¼­ Áß¿äÇÑ ¿ªÇÒÀ» ¼öÇàÇϸç, Ư¡¦
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