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  • cell culture and MTT assay   (1 ÆäÀÌÁö)
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cell culture and MTT assay

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ÆÄÀÏ : cell culture and MTT assay.hwp   [Size : 62 Kbyte ]
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1)-1 Sub Culture

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1.1 Cell

1.2 Cell subculture

1.3 Cell media

1.4 Cryopreservation

1)-2 MTT assayÀÇ ¿ø¸®

2) ½ÇÇèÀÇ ¿ª»ç

2)-1 Sub Culture ½ÇÇèÀÇ ¿ª»ç
1.1 ¼¼Æ÷Á¶Á÷¹è¾çÀÇ ¿ª»ç
1.2 EagleÀÇ ½ÇÇè
2. Material
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2) µµ±¸
3. Method
1) ½ÇÇè¹æ¹ý
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1)-1 Sub Culture

1)-2 MTT assay
2) ÁÖÀÇ»çÇ×
4. Result
5. Discussion
6. Reference
1. Introduction

¼¼Æ÷ ¹è¾ç(Cell Culture)°ú MTT ºÐ¼®(MTT Assay)Àº »ý¸í°úÇÐ ¹× ÀÇÇÐ ¿¬±¸¿¡¼­ Áß¿äÇÑ ±â¹ýÀ¸·Î ÀÚ¸®Àâ°í ÀÖ´Ù. ¼¼Æ÷ ¹è¾çÀº »ì¾ÆÀÖ´Â ¼¼Æ÷¸¦ ÀΰøÀûÀΠȯ°æ¿¡¼­ ¼ºÀå½ÃÅ°°í À¯ÁöÇÏ´Â ±â¼ú·Î, ÁÖ·Î ¿¬±¸½Ç¿¡¼­ ƯÁ¤ ¼¼Æ÷ÁÖ¸¦ °¡Áö°í ½ÇÇèÀ» ÁøÇàÇÒ ¶§ »ç¿ëµÈ´Ù. ÀÌ ¹æ¹ýÀ» ÅëÇØ ¿¬±¸ÀÚµéÀº ¼¼Æ÷ÀÇ »ý¸®Àû, »ýÈ­ÇÐÀû Ư¼ºÀ» ¿¬±¸ÇÏ°í, ´Ù¾çÇÑ ¾à¹°À̳ª È­ÇÕ¹°ÀÌ ¼¼Æ÷¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Æò°¡ÇÒ ¼ö ÀÖ´Ù. ¼¼Æ÷ ¹è¾çÀÇ ¿ì¼ö¼ºÀº ¼¼Æ÷°¡ »ýü ³» ȯ°æ°ú À¯»çÇÑ Á¶°Ç¿¡¼­ ÀÚ¶ó°í ¹ø½ÄÇÒ ¼ö ÀÖ´Ù´Â Á¡¿¡ ÀÖÀ¸¸ç, À̸¦ ÅëÇØ ¼¼Æ÷ °£ÀÇ »óÈ£ÀÛ¿ë, ½ÅÈ£ Àü´Þ °æ·Î ¹× À¯ÀüÀÚÀÇ ¹ßÇö µî ´Ù¾çÇÑ »ý¹°ÇÐÀû °úÁ¤À» ½ÉÃþÀûÀ¸·Î ÀÌÇØÇÒ ¼ö ÀÖ´Ù. MTT ºÐ¼®Àº ¼¼Æ÷ »ýÁ¸¼º°ú Áõ½Ä ´É·ÂÀ» Æò°¡ÇÏ´Â µ¥ ³Î¸® »ç¿ëµÇ´Â »ö¼ÒÈ­ÇÕ¹° ±â¹ÝÀÇ ¹æ¹ýÀÌ´Ù. ÀÌ ºÐ¼®¹ýÀº ¼¼Æ÷°¡ »ì¾ÆÀÖÀ» ¶§ ƯÁ¤ È¿¼Ò¿¡ ÀÇÇØ È¯¿øµÇ´Â MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)¸¦ ÀÌ¿ëÇÏ¿©, ¼¼Æ÷ÀÇ ´ë»çÈ°µ¿À» Á¤·®ÀûÀ¸·Î ÃøÁ¤ÇÏ´Â ¿ø¸®¸¦ °¡Áö°í ÀÖ´Ù. ¼¼Æ÷°¡ »ì¾ÆÀÖÀ» ¡¦(»ý·«)


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ID : hajo******
Regist : 2024-10-12
Update : 2024-10-12
FileNo : 25877073

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cell   culture   MTT   assay  


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