1. Abstract
Phenol red, a pH indicator commonly used in various biological and chemical applications, exhibits a notable temperature-dependent behavior in its dissociation constant when dissolved in phosphate buffers. This study explores the intricate relationship between temperature variations and the dissociation constant of phenol red through meticulous deconvolution of UV-Vis spectra, enabling a detailed understanding of the underlying chemical dynamics. As temperature increases, the ionization state of phenol red shifts, thus affecting the equilibria of the protonated and deprotonated forms. The dissociation constant, which defines this equilibrium, can consequently change, highlighting the importance of considering temperature in applications involving this pH indicator. The deconvolution of UV-Vis spectra serves as a powerful analytical technique for unraveling these temperature-induced variations, allowing for precise differentiation between the absorbance characteristics
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