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寃곌낵�젅�룷�듃
�떎 �뿕 �젣 紐� :
Liquid culture & Expression
議� :
�븰 踰� :
�씠 由� :
1. Abstract
�씠踰� �떎�뿕��� �븸泥퀽B諛곗���뿉 eGFP DNA媛� �룷�븿�릺�뼱 �엳�뒗 �떎�뿕援곌낵 �룷�븿�릺�뼱 �엳吏� �븡�뒗 ���議곌뎔�쓣 諛곗뼇�떆�궓 �뮘 �몢 �뵆�씪�뒪�겕�뿉 �떒諛깆쭏諛쒗쁽 �쑀�룄臾쇱쭏�씤 IPTG瑜� �꽔�뼱 諛곗뼇�떆�궎怨�, UV transilluminator瑜� �끃�깋 �삎愿묐튆�쓽 諛쒗쁽�쓣 愿�李고븯�뒗 �떎�뿕�씠�뿀�떎. �떎�뿕�쓽 寃곌낵, eGFP DNA媛� �룷�븿�릺�뼱 �엳吏� �븡��� ���議곌뎔�뿉�꽌�뒗 �끃�깋�쓽 �삎愿묐튆�씠 愿�李곕릺吏� �븡��� 諛섎㈃ eGFP DNA瑜� �꽔�뼱以� �떎�뿕援곗뿉�꽌�뒗 紐⑤뱺 議곗뿉�꽌 �끃�깋�쓽 �삎愿묐튆�씠 愿�李곕릺�뿀�떎. �씠�젃寃� eGFP �쑀�쟾�옄瑜� 諛쒗쁽�떆�궎�뒗 Lac operon�쓽 system�뿉 ����빐�꽌 �븣�븘蹂닿퀬, Cell growth curve��� O.D.�쓽 愿�怨꾩뿉 ����빐�꽌�룄 遺꾩꽍�빐蹂댁븯�떎.
2. Experiment
1) �떎�뿕 湲곌린
(1) Autoclave (怨좎븬硫멸퇏湲�)
- 硫�
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