±× ¿¹·Î, APAFÀÇ Chiron Project¸¦ µé¾îº¸¸é µ¿ÀÏÇÑ ¾Ï ȯÀÚÀÇ °æ¿ì¿¡µµ ÀüÀÌ°¡ »¡¸® ÁøÇàµÈ °æ¿ìÀÇ È¯ÀÚ¿Í ÀüÀÌ°¡ ¾ÈµÈ ȯÀÚÀÇ ¼¼Æ÷ÁÖ¿¡¼ ¾òÀº chironÀ¸·Î 219°³ÀÇ protein spotÀ» ºÐ¼®ÇÏ¿´´Ù. ±× °á°ú, 205°³ÀÇ spotÀº ÀÌ¹Ì ¾Ë·ÁÁø ´Ü¹éÁú À̾ú°í, 14°³ÀÇ »õ·Î¿î protein spotÀ» ã¾Æ³Â´Ù. ÀÌµé µÎ ½Ã·áÀÇ Â÷ÀÌÁ¡Àº 125°³ÀÇ ´Ù¸¥ protein spotÀ» ã¾Æ ³ÂÀ¸¸ç, À̵éÀº ´ëºÎºÐÀÌ phosphorylation ¹× glycosylationµîÀÇ post-translational modification¿¡ ÀÇÇÑ °ÍÀ¸·Î ¹àÇôÁ³´Ù. ±×¸®°í ¼·Î ´Ù¸£°Ô ¹ßÇöµÈ 25°³ÀÇ ´Ü¹éÁúÀ» ¾Ë¾Æ³Â°í, metastasis¿Í °ü·ÃµÈ 2°³ÀÇ ´Ü¹éÁú, cancer¿Í °ü·ÃµÈ 18°³ÀÇ ´Ü¹éÁú µîÀ» ¾Ë¾Æ³¾ ¼ö ÀÖ¾ú´Ù. ¶ÇÇÑ ÀÌµé »õ·Î¿î ´Ü¹éÁú Áß 4°³´Â heat shock proteinÀ» Æ÷ÇÔÇÑ phosphoproteinÀÓÀ» ¾Ë¾Æ³Â´Ù.
3) Proteomics ¹ß´ÞÀÇ °è±â
DNAÁõÆø±â¼ú(PCR)ÀÌ GenomicsºÎ¹®¿¡¼ Áö³ð ±¸Á¶ °áÁ¤ÀÇ ÀüȯÁ¡ÀÌ µÉ¸¸ÇÑ ±â¼úÀ̾ú´ø °Íó·³, À¯ÀüÀÚ ¿¬±¸¿¡ ºñÇÏ¿© ºÐ¸® ¹× Á¤Á¦ ¹®Á¦ µîÀÇ ¾î·Á¿òÀ» °Þ´ø ´Ü¹éÁú ¿¬±¸´Â 2-D gel electrophoresis ±â¼ú°ú MALDI MS ºÐ¼® ±â¼úÀÇ ¹ß´ÞÀ» °è±â·Î ´ë·® È¿´É °Ë»ö(High Through put Screening : HTS) ±â´ÉÀ» È®º¸¡¦(»ý·«)
¨ç Sample preparation
¨è 2-D Electrophoresis
¨é Staining
¨ê Image Acquisition
¨ë Image Analysis
¨ì Protein Excision
¨í Protein Identification
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1) http://kin.naver.com/open100/entry.php?docid=46372 2) http://kin.naver.com/browse/db_detail.php?d1id=7&dir_id=709&docid=35478 3) Proteomics ±â¼úÀÇ °³¹ß ¹× ÀÀ¿ë, ÀÌÀº±Ô ¿Ü 2ÀÎ, Çѱ¹»ý¹°°øÇÐȸÁö, 2002. 4) Proteomics : »õ·Î¿î ±â¼ú°ú ÀÀ¿ë, Çѱ¹»ý¹°°øÇÐȸ, ÀÌ»óö, 2001. 5) MS¸¦ ÀÌ¿ëÇÑ Á¤·®Àû proteomics, »ýÈÇдº½º, ±èÁø¼ö, 1999
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