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Kim, Il Hae
The Phylogeny of Land Plants
Inferred from 18s rDNA Sequences :
Pushing the Limits of rDNA Signal
Kim, Hyun Jin
Kim, Yun Jong
Jeon, Sang Hyun
Jo, Yun Na
Lee, Sang Min
O, Tae Hyun
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Table of Contents
Introduction
Materials & Methods
Results
Discussion
Conclusion
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Part I
- Jo, Yun Na
Introduction
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Introduction
Unclear relationship of many of the major lineages of land plants for understanding land plant phylogeny
Ribosomal RNA or DNA sequences are used to reconstruct deep branches of evolutionary history
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INTRODUCTION
What is 18S rRNA
A part of the ribosomal RNA
A component of the small eukaryotic ribosomal subunit (40S)
The structural RNA for the small component of eukaryotic cytoplasmic ribosomes - one of the ba¡¦(»ý·«)
1) A phylogenetic analysis of complete 18S rDNA sequences for 93 species of land plants
2) The usefulness of 18S rDNA sequences for reconstructing deep branched of plant phylogeny
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: http://www.wsu.edu:8080/~soltilab/.
Outgroups : Sequence of (1) coleochaetales & (2) charales
Amplication and sequencing of 18S rDNA
Both manual and automated sequencing methods were used
MATERIALS AND METHODS
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MATERIALS AND METHODS
Aligned by eye : easy to align across all land plants except the bryophytes
Aligned sequence : 1.869 bp in length
Remaining ambiguous alignment areas and terminal priming sites(130bp) available at - wsu.edu and the - Green Plant Phylogeny Research Coordination Group
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MATERIALS AND METHODS
Heuristic parsimony analyses: PAUP 4.0
RANDOM taxon addition & TBR branch swapping: first 200 replicates
saved 10 most parsimonious trees per replicate
10 shortest trees of 2,787 steps
:used as starting trees (via TBR branch swapping & saving all MULPARS)
1,883 trees from the initial 200 replicate searches: used as starting tree to find shorter tree
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