REPORT OF LABORATORY EXPERIMENTS IN ORGANIC CHEMISTRY ¨ç
1. Subject : Thin Layer Chromatography (TLC)
2. Date :
3. Name : -
4. Principle :
Non-volatile reaction mixtureÀÇ qualitative examination¿¡ »ç¿ëÀÌ µÇ´Â simpleÇÏ°í rapidÇÑ ÀÏÁ¾ÀÇ liquid chromatograghy·Î glass, aluminum ¶Ç´Â plastic plate¿¡ solid stationary phase°¡ coatingµÇ¾îÀÖ´Ù.
¿ø¸®´Â ºÐ¸®ÇÏ°íÀÚ ÇÏ´Â ¹°ÁúÀÇ mobile phase¿Í stationary phase¿¡ ´ëÇÑ adsorptionÁ¤µµ¿¡ µû¶ó ¹°ÁúÀÌ ºÐ¸®µÈ´Ù.
(1) »ç¿ëµÇ´Â °æ¿ì
- Sample purification monitoring
- Quick establishment of optimum conditions for carrying out a synthesis
- Examination of crude reaction mixture
- Determining the homogeneity of a sample
- Conjunction with other evidence
- Establishing whether or not samples from different sources are identical
¢¡ Qualitative analysis of quantities as small as 10 microgram
(2) Adsorbent (Stationary phases)
- Silica gel, alumina, kieselguhr, cellulose powder
- Fluorescent compound¿¡ »ç¿ëÀÌ °¡´ÉÇϸç mixture compoundÀÇ resolut¡¦(»ý·«)
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